TY - Jour A2 - Kopecki，Zlatko Au - Lu，海元奥 - 王，粉丝·梅，华AU - 王，Siqi Au - Cheng，Lamei Py - 2018 DA - 2018/12/13 Ti - 人类脂肪型间充质干细胞在脐带和子宫内膜SP - 7537589 VL-2018 AB - 血管生成是一种更高效的血管生成促进促进促进内皮菌落形成细胞促进促进 - 血管生成是一种复杂的过程，其中大脑细胞发挥重要作用。已经证明了来自不同组织的多能的间充质细胞/基质细胞（MSCs）是具有血管外细胞的致致生成的和份子表达谱。然而，不同的组织衍生的MSCs可以表现出临床应用的不同潜力。因此，对不同MSCs的比较研究是必不可少的。在这里，我们以表面抗原表达，分化能力和血管生成促进在内皮菌落 - 形成细胞（ECFC）上的血管生成促进的能力方面的特征在于体外和体内。观察到免疫蛋白型和分化无显着差异。另外，三种类型的MSCS，遍布由Matrogel上的Coculture系统中的ECFC形成的管状结构周围。但是，在Adscs播种的ECFCs Monolayer形成了比UCMSC或EMSC在UCMSCs或Emscs上的更有组织的毛细管样网络。 When suspended with ECFCs in matrigel and implanted into nude mice, ADSCs promoted more functional vessel formation after 7 days. Moreover, in murine hindlimb ischemia model, cotransplantation of ECFCs with ADSCs was significantly superior to UCMSCs and EMSCs in promoting perfusion recovery and limb salvage. Furthermore, ADSC-conditioned medium (CM) contained more proangiogenic factors (such as vascular endothelial growth factor-A, platelet-derived growth factor BB, and basic fibroblast growth factor) and less inhibitory factor (such as thrombospondin-1), when compared with UCMSC-CM and EMSC-CM. And ADSC-CM more durably stabilized the vascular-like structures formed by ECFCs on matrigel and promoted ECFCs migration more efficiently. In summary, MSCs from adipose show significantly efficient promotion on angiogenesis both in vitro and in vivo than UCMSCs and EMSCs. Hence, ADSCs may be recommended as a more suitable source for treating hindlimb ischemia. SN - 1687-966X UR - https://doi.org/10.1155/2018/7537589 DO - 10.1155/2018/7537589 JF - Stem Cells International PB - Hindawi KW - ER -