TY - JOUR A2 - 夏，雨辰AU - 张，Yongting AU - 李，宇文AU - 李闻听AU - 蔡杰盟 - 悦明AU - 江，龙凤非盟 - 徐蕊蕊AU - 张，丽丽AU -李，军盟 - 朱川隆PY - 2018 DA - 2018年11月14日TI - 脐的治疗作用脐血间充质干在对急性肝衰竭各代细胞在大鼠SP - 7159465 VL - 2018 AB - 最近的研究描述beneficial effects of an infusion of mesenchymal stem cells (MSCs) derived from Wharton’s jelly tissue, for the treatment of acute liver failure (ALF). However, data on the therapeutic potential of culture-expanded MSCs are lacking. We examined the therapeutic potential of passage five (P5) and ten (P10) human umbilical cord- (hUC-) MSCs via their transplantation into Sprague-Dawley (SD) rats with D-galactosamine (D-GalN) and LPS-induced acute liver failure (ALF). SD rats were randomly divided into three groups: control group, P5 hUC-MSCs group, and P10 hUC-MSCs group. After transplantation, P5 hUC-MSCs provided a significant survival benefit. The analysis of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBIL) levels showed that transplantation with P5 hUC-MSCs was more effective than treatment with P10 hUC-MSCs. P5 hUC-MSCs also successfully downregulated the hepatic activity index (HAI) scores. Compared to P10 hUC-MSCs 体内，P5 HUC-MSC的显著增强再生和抑制肝细胞的细胞凋亡。CM-DiI标记HUC-MSC的发现给收件人肝脏内嫁接，而细胞的归巢于P10相比，P5 HUC-MSCs组HUC-MSCs组是不太有效的接收者肝脏。以前的研究已经表明，肝细胞生长因子（HGF）在伤肝浓度显著上升。HGF通常被称为c-Met的配体。c-Met的在HUC-MSC的通过Western印迹检测到的水平表明，在较高的传代次数，存在的c-Met的减少。这些数据表明P5 HUC-MSC的是直接移植可以更有效地回家了伤肝。随后，P5 HUC-MSC的可以拯救ALF并通过内源性肝再生和肝细胞凋亡为补偿肝功能，这是依赖于比P10 HUC-MSC的更高级别的c-Met的抑制的刺激重新填充大鼠肝脏。SN - 1687-966X UR - https://doi.org/10.1155/2018/7159465 DO - 10.1155 /七百一十五万九千四百六十五分之二千○十八JF - 干细胞国际PB - Hindawi出版KW - ER -