TY - JOUR A2 - Sorrenti, Valeria AU - Reppe, Sjur AU - Jackson, Catherine Joan AU - Ringstad, Håkon AU - Tønseth, Kim Alexander AU - Bakke, Hege AU - Eidet, Jon Roger AU - Utheim，Tor Paaske PY - 2018 DA - 2018/11/18 TI -高通量筛选的添加剂使用阶乘设计,促进生存的动物上皮表存储SP - 6545876六世- 2018 AB -需要优化存储条件保存细胞培养细胞表从专业文化的特点在运输单位遥远的医院。在本研究中，我们旨在探索一种方法，使用多因素设计和自动化筛选程序，以确定能够降低未分化表皮细胞存活率的添加剂。培养的细胞在12°C加各种添加剂的培养基中保存7-11天。通过钙黄素对活细胞的染色和形态学评价其作用。26种添加剂采用(1)两水平析因设计，在64种不同组合中添加或省略10种添加剂;(2)混合设计，在总共64种不同混合物中添加5种不同浓度的5种添加剂。自动显微镜和细胞计数与斐济使数据的有效处理。通过Design-Expert软件确定显著回归模型。在6%甘油的情况下，细胞片存储11天，活细胞的最大增加达到37±6%。 The beneficial effect of glycerol was shown for epidermal cell sheets from three different donors in two different storage media and with two different factorial designs. We have thus developed a high throughput screening system enabling robust assessment of live cells and identified glycerol as a beneficial additive that has a positive effect on epidermal cell sheet upon storage at 12°C. We believe this method could be of use in other cell culture optimization strategies where a large number of conditions are compared for their effect on cell viability or other quantifiable dependent variables. SN - 1687-966X UR - https://doi.org/10.1155/2018/6545876 DO - 10.1155/2018/6545876 JF - Stem Cells International PB - Hindawi KW - ER -