TY -的A2南Hyun盟——Alekseenko l . l . AU - Shilina m . a . AU - Lyublinskaya o . g . AU - Kornienko j . s . AU - Anatskaya o . v . AU - Vinogradov, a . e . AU - Grinchuk t . m . AU - Fridlyanskaya i . i . AU - Nikolsky,技术文库>资源分类>行业论文>医学论文> quiet - escent Human Mesenchymal Stem Cells Are More Resistant to Heat Stress than Cycling Cells然而，令人惊讶的是，我们对静止细胞如何应对环境挑战知之甚少。本研究的目的是比较循环和静态间充质干细胞(MSC)的应激反应。采用人子宫内膜间充质细胞(eMSС)作为成体干细胞。eMSC静止采用血清饥饿模拟。亚致死热休克(HS)作为应激因子。静息细胞和循环细胞均在45°C下加热30分钟，然后返回标准培养条件进行恢复。HS反应通过DNA损伤反应、应激诱导的早衰(SIPS)、细胞增殖活性和氧化代谢来监测。研究发现，与增殖细胞相比，静止细胞修复DNA更快，恢复增殖，发生SIPS更少。 HS-enforced ROS production in heated cycling cells was accompanied with increased expression of genes regulating redox-active proteins. Quiescent cells exposed to HS did not intensify the ROS production, and genes involved in antioxidant defense were mostly silent. Altogether, the results have shown that quiescent cells are more resistant to heat stress than cycling cells. Next-generation sequencing (NGS) demonstrates that HS-survived cells retain differentiation capacity and do not exhibit signs of spontaneous transformation. SN - 1687-966X UR - https://doi.org/10.1155/2018/3753547 DO - 10.1155/2018/3753547 JF - Stem Cells International PB - Hindawi KW - ER -