TY -的A2 Gronthos斯坦盟——刘、张盟-罗,京盟——张Ke-ke AU -林,龙翔盟——梁、Ting AU -罗,Zong-ping盟,壮族Yong-qing盟——太阳,yu long PY - 2018 DA - 2018/10/28 TI - Tendon-Derived退行性肌腱干细胞分化的微环境SP - 2613821六世- 2018 AB -病变是流行的运动,很多职业人群;然而,病变的发病机制仍不清楚。Tendon-derived干细胞(TDSCs)被认为是罪魁祸首对于腱子病变衰退的发展的关键。然而,它是不确定如何TDSCs分化成脂肪细胞、软骨细胞、骨细胞退行性微环境的病变。因此,在这项研究中,退行性肌腱分化的微环境的调节影响TDSCs进行调查。TDSCs被孤立的从大鼠跟腱和生长在正常和退行性(由stress-deprived文化)脱细胞肌腱片(dts)。免疫荧光染色,圆)染色、实时PCR和免疫印迹被用来描绘形态、增殖、分化的TDSCs退行性微环境。发现TDSCs更蔓延在退行性dts比正常的dts。tenocyte-related标记,COL1和TNMD高度表达正常比退行性dts dts。chondrogenic和成骨的标记的表达、COL2 SOX9、Runx2,高山,在退行性dts与TDSCs相比正常的dts。 Furthermore, phosphorylated FAK and ERK1/2 were reduced on degenerative DTSs. In conclusion, this study found that the degenerative tendon microenvironment induced TDSCs to differentiate into chondrogenic and osteogenic lineages. It could be attributed to the cell morphology changes and reduced FAK and ERK1/2 activation in the degenerative microenvironment of tendinopathy. SN - 1687-966X UR - https://doi.org/10.1155/2018/2613821 DO - 10.1155/2018/2613821 JF - Stem Cells International PB - Hindawi KW - ER -