Tao, Kai AU - Han, Xuesong AU - Zhuang, Yanfeng AU - Zhang, Zhihong AU - Guo, Lin AU - Wang，Wanming PY - 2016 DA - 2016/11/22 TI -监管机制的本次事件/ PTHrP信号通路在Fibrochondrocytes Entheses猪跟腱SP - 8235172六世- 2016 AB -本研究旨在探索压力刺激增殖和分化的影响通过印度Fibrochondrocytes Entheses介导的hedgehog (Ihh)/甲状旁腺激素相关蛋白(PTHrP)信号通路。对纤维软骨细胞施加不同的应激刺激。检测纤维软骨细胞胞浆中I型胶原蛋白(Col I)、Col II、Col X、Ihh、PTHrP等信号分子的基因表达及蛋白水平。采用Ihh信号通路特异性阻断剂环巴胺建立Ihh信号阻断组。用PTHrP试剂建立PTHrP增强组。纳入Ihh/PTHrP双重干预组和对照组，研究Ihh/PTHrP信号通路在纤维软骨细胞中的调控机制。低循环应力拉伸(CTS)下，PTHrP、Col I和Col II基因表达和蛋白合成增加。在高CTS下，Ihh和Col X基因表达和蛋白合成增加。 Blocking Ihh signaling with cyclopamine resulted in reduced PTHrP gene expression and protein synthesis and increased Col X gene expression and protein synthesis. Ihh and PTHrP coregulate fibrochondrocyte proliferation and differentiation in entheses through negative feedback regulation. Fibrochondrocyte is affected by the CTS. This phenomenon is regulated by stress stimulation through the Ihh/PTHrP signaling pathway. SN - 1687-966X UR - https://doi.org/10.1155/2016/8235172 DO - 10.1155/2016/8235172 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -