TY - JOUR A2 - Brunelli, Silvia AU - Nagaria, Pratik K. AU - Robert, Carine AU - Park, Tea Soon AU - Huo, Jeffrey S. AU - Zambidis, Elias T. AU - Rassool，Feyruz v . PY - 2016 DA - 2016/09/01 TI -高保真重组人类细胞则有很高的DNA修复的功效,像为MYC基因转录签名SP - 3826249六世- 2016 AB -人类诱导多能干细胞(hiPSCs)从成年或前体细胞重编程和必须大量适应保证genomic stability in order to become “embryonic stem cell- (ESC-) like.” The DNA damage response (DDR) is critical for maintenance of such genomic integrity. Herein, we determined whether cell of origin and reprogramming method influence the DDR of hiPSCs. We demonstrate that hiPSCs derived from cord blood (CB) myeloid progenitors (i.e., CB-iPSC) via an efficient high-fidelity stromal-activated (sa) method closely resembled hESCs in DNA repair gene expression signature and irradiation-induced DDR, relative to hiPSCs generated from CB or fibroblasts via standard methods. Furthermore, sa-CB-iPSCs also more closely resembled hESCs in accuracy of nonhomologous end joining (NHEJ), DNA double-strand break (DSB) repair, and C-MYC transcriptional signatures, relative to standard hiPSCs. Our data suggests that hiPSCs derived via more efficient reprogramming methods possess more hESC-like activated MYC signatures and DDR signaling. Thus, an authentic MYC molecular signature may serve as an important biomarker in characterizing the genomic integrity in hiPSCs. SN - 1687-966X UR - https://doi.org/10.1155/2016/3826249 DO - 10.1155/2016/3826249 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -