TY -的A2 D 'Elios,马里奥•m . AU - Gu Yeun-Hwa盟,山下式Takenori盟——井上,完全的盟歌,Jin-Ho AU - Kang Ki-Mun PY - 2019 DA - 2019/04/14 TI -细胞和分子水平机制对电化学治疗癌症SP - 3431674六世- 2019 AB -电化学治疗(ECT)是一种很有前途的新方法诱导肿瘤回归通过直流电流入癌组织。在临床研究中，ECT应用于不同类型的肿瘤均取得了良好的效果。此外，在疗效评价、剂量反应和细胞毒性方面的基础研究也几乎没有。因此，研究ECT抗肿瘤作用的细胞机制，为ECT的基础研究提供数据。在细胞水平的研究中，使用ICR小鼠和C3H小鼠对肿瘤细胞(肉瘤-180、Scc-7、Ehrlich癌)进行了研究。研究组分别测量对照组、10mA×150s组、10mA×300s组和10mA×600s组pH值5次。在组织学水平的研究中，对C3H小鼠右脚上部接种的肿瘤进行ECT。每组在电休克治疗后6、12、24小时行颈椎脱位处死小鼠，并切除肿瘤。将切除的肿瘤用10%福尔马林固定在组织中，从组织切片上观察得到的HE染色和凋亡抗体染色。在细胞水平的研究中，在肿瘤生长测量研究中，与对照组相比，所有ECT治疗组的肉瘤患者均有统计学差异。 Statistically significant differences were also observed in Scc-7 in all ECT groups compared to the control group. In the intratumoral pH measurement study, there was a statistically significant difference between the anode and the cathode in each group compared to the control group. In the examination at the histological level, microscopic observation of a slide stained with apoptosis antibody with a magnification of 400 times showed that 6hrs after ECT it was stronger and then decreased. By performing ECT, a weak current flows in the living body. As a result, changes in tissue pH, generation of gas, etc. occur. In this study, it was also confirmed that the intratumor pH value becomes strongly acidic on the anode side and strongly alkaline on the cathode side. In addition, this study confirmed the occurrence of gas during treatment of ECT. Changes in the pH and the like cause changes in the environment in the cell, denaturation of proteins, apoptosis, and necrosis. In this study, a significant increase in apoptosis was confirmed in each ECT group compared to the control group. Treatment effects by ECT were also observed in tumor growth measurement studies and tumor weight measurement studies. From these research results, ECT is considered to be effective as a tumor treatment method. SN - 2090-3057 UR - https://doi.org/10.1155/2019/3431674 DO - 10.1155/2019/3431674 JF - Journal of Pathogens PB - Hindawi KW - ER -