ty -jour a2 -berkhout，ben au -scoville，delia J. au -uhm，tae kyu brian au -shallcross，Jamie A. Au -Whelan，Rebecca J. Py -2017 da -2017/02/09 TI-选择DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing SP - 9879135 VL - 2017 AB - CA125 is a mucin glycoprotein whose concentration in serum correlates with a woman’s risk of developing ovarian cancer and also indicates response to therapy in diagnosed patients. Accurate detection of this large, complex protein in patient samples is of great clinical relevance. We suggest that powerful new diagnostic tools may be enabled by the development of nucleic acid aptamers with affinity for CA125. Here, we report on our use of One-Pot SELEX to isolate single-stranded DNA aptamers with affinity for CA125, followed by high-throughput sequencing of the selected oligonucleotides. This data-rich approach, combined with bioinformatics tools, enabled the entire selection process to be characterized. Using fluorescence anisotropy and affinity probe capillary electrophoresis, the binding affinities of four aptamer candidates were evaluated. Two aptamers, CA125_1 and CA125_12, both without primers, were found to bind to clinically relevant concentrations of the protein target. Binding was differently influenced by the presence of Mg²⁺离子，结合Ca125_1并废除CA125_12的结合所需。总之，发现一锅SELEX是一种有前途的选择方法，它将DNA适体用于临床上重要的蛋白质靶标。SN -2090-0201 UR -https：//doi.org/10.1155/2017/9879135 do -10.1155/2017/2017/9879135 JF-核酸杂志PB- Hindawi KW -er -er-