TY - JOUR A2 - Cozzolino, Daniel AU - Nganou, N. D. AU - Tchinda, E. S. AU - Noumo, T. N. AU - Mouafo, H. T. AU - Sokamte, A. T. AU - Tatsadjieu, L. N. PY - 2020 DA - 2020/10/12 TI - Fungal Diversity and Evaluation of Ochratoxin A Content of Coffee from Three Cameroonian Regions SP - 8884514 VL - 2020 AB - The present study had the objective to assess the ochratoxin A content of coffee through chromatographic analysis and design a method using PCR-DGGE to analyze at the same moment the totality of fungal flora present in the coffee samples in order to determine their geographic origin. 96 samples of coffee were collected from the west region (Bafoussam and Dschang), centre region (Bafia), and east region (Batouri) of Cameroon during two years (2017 and 2018). Two treatments (dry and wet routes) were evaluated at three different steps of coffee processing (parchment coffee, green coffee, and husk coffee). The characterization of the fungal profile was done with PCR-DGGE and sequencing. The levels of OTA were assessed using HPLC analysis. The results indicated that the toxinogenic mycoflora associated with coffee beans was mainly Aspergillus尼日尔那 A. Carbonarius，和 A. Ochraceus.。PCR-DGGE数据显示，每个采样部位的特征在于特定的真菌轮廓。尽管治疗对真菌群咖啡群的影响，但观察到来自同一部位的样品共同的乐队。因此，这些带构成潜在的生物标志物，以追溯到咖啡的起源。在大多数咖啡样本中检测到OTA，分析，只有少量的样品含量高于含量高于用于人类消费的食物的最大可耐受限额。咖啡的OTA含量受到抽样步骤和采样期的显着影响。SN - 0146-9428 UR - https://doi.org/10.1155/2020/8884514 Do - 10.1155 / 2020/8884514 JF - 食品质量PB - Hindawi KW - ER -