TY - JOUR A2 - 罗维拉，霍尔迪AU - 潘，演译AU - 秋，德意AU - 陈，建AU - 悦，巧云PY - 2020 DA - 2020年4月9日TI - 结合了COI迷你条码与新一代测序动物来源成分鉴定加工肉制品SP - 2907670 VL - 2020 AB - 对于复杂的暴露动物物种或掺假加工肉制品中，我们提出的方法相结合的新的细胞色素氧化酶I（ COI）迷你条形码与下一代测序（NGS），其识别各种动物（猪，牛，羊亚科，有的鱼，虾，和家禽）准确且高效地在被处理的肉产品。我们设计了一种基于51个可食性动物种类140个序列的通用引物。A mixture of 12 species raw meat samples were identified with the clone sequencing and also with a mini-barcode- (136 bp) combined NGS method, respectively. The mini-barcode of these 12 species was 100% identical to the target species sequence by Sanger sequencing. Compared to the clone sequencing method, the NGS method is superior in accuracy, sensitivity, and detection efficiency. Various edible animal species were identified in the species level both in the mixed samples and the 7 heavily processed food products. Moreover, some 未标记的种类和可疑的污染是检测为好。SN - 0146-9428 UR - https://doi.org/10.1155/2020/2907670 DO - 10.1155 /二百九十○万七千六百七十○分之二千零二十JF - Hindawi出版KW - - ER中国食品质量PB的 -