化学分析方法杂志

挥发性成分Haplophyllum canaliculatum羌活。在伊朗生长通过水蒸气蒸馏（HD），无溶剂微波萃取（SFME）和顶空固相微萃取（HS-SPME）萃取。的成分用GC和GC-MS进行分析。对于HS-SPME，微波，功率和萃取SFME的曝光时间的提取时间和温度进行了优化。由HD方法获得二十五个化合物表示在油总化合物的99.88％，而此方法的主要成分被鉴定为β-蒎烯（18.90%）、1,8-桉叶素（13.94%）和哌啶酮（12.22%）。然而，哌啶酮（34.50%）、石竹烯氧化物（9.94%）和一个-eudesmol是主要的化合物中21的组分，表示进行了表征在由SFME法提取挥发物总组合物的99.89％。此外，13个的化合物，占总组分的99.95％，进行了表征在由HS-SPME方法，这是由主导提取挥发性馏分β-蒎烯（21.13%），一个蒎烯（13.07％），苎烯（11.65％），和δ-2-蒈烯（10.23％），为主要成分。

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环保，快速，成本提高两地的分光光度法进行了开发和验证，阿替洛尔，对乙酰氨基酚，氢氯噻嗪，和左氧氟沙星的决心。第一种方法是新开发的扩展衍生物比（EDR）和第二方法是多变量曲线交替分辨率最小二乘（MCR-ALS）。In the EDR method, the extended derivative ratio amplitudes at 281.6, 237.6, 279.2, and 282.8 nm were used for quantification of atenolol, paracetamol, hydrochlorothiazide, and levofloxacin, respectively. In the MCR-ALS method, calibration model was developed and correlation constraint was employed. External validation data set composed of seven mixtures was used, and different figures of merits such as root mean square error of prediction, standard error of prediction, bias, and relative error of prediction were calculated, and satisfactory results were obtained. Both methods provided comparable results. The methods were validated and applied for the determination of the target analytes in dosage forms, spiked and real human urine. Thereafter, the obtained results were statistically compared to the published methods and revealed no significant difference regarding accuracy and precision. Furthermore, the greenness profile of the methods was evaluated using the National Environmental Methods Index “NEMI” and Analytical Eco-Scale. The developed methods can be used as a valid eco-friendly and simple cost-effective alternative to the commonly used chromatographic methods for the routine analysis of the studied drugs in dosage forms and human urine.

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法拉花（FF）在我国已有数千年的广泛应用，但其药动学特性却鲜为人知。本研究旨在建立一种快速、准确的超高效液相色谱-三联四极杆串联质谱法，比较生粉和蜜粉提取物口服后8种活性成分的药动学研究。在热Hypersil GOLD C18柱（100 mm × 2.1 mm，1.9 ）上进行了最佳分离µm particles size) with a gradient elution of acetonitrile as mobile phase A and 0.3% formic acid aqueous solution as mobile phase B. The flow rate was set as 0.3 mL/min and separated for 34.0 minutes. Electrospray ionization in the negative ion mode and selected reaction monitoring were used to identify and separate active components. The results met the acceptance criteria and showed that this method exhibited good linear, precision, accuracy, and stability. The extraction recoveries ranged from 81.54% to 104.48%, and the matrix effects ranged from 81.94% to 103.02%. These results show that the validated method could be successfully applied to evaluate the pharmacokinetic study in rats after oral administration of raw farfarae flos (R-FF) and honey-processed farfarae flos (H-FF).

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在这项工作中，通过激光烧蚀锆石的高空间分辨率的U-Pb定的新的方法在电感10-16耦合等离子体质谱法（LA-ICP-MS） μ米光斑直径已经提出。这是通过引入2％（达到 ）水 - 乙醇蒸气到ICP与屏蔽炬系统增加的灵敏度和抑制同位素分馏效应组合。使用所提出的方法，获得精确和准确的一致U-Pb年龄为Plešovice，GJ-1，和91500个锆石，其结果与同位素稀释热电离质谱（ID-TIMS）和LA-ICP-MS吻合在2σ，除了年龄使用干等离子体获得的10 μm点直径。另外，等离子体条件的影响（干等离子体或2%( ）乙醇血浆）和斑点大小（10、16、24或32 μ上精度米）（RSD），准确性（RE），和不确定性（2σ）的^206个铅/²³⁸ü年龄进行了研究。结果表明，提高了光点直径，并引入2％（ ）水 - 乙醇蒸气进入ICP显著提高了精度，准确度和不确定性对于小光斑直径（10和16 μm） 对中间光斑直径（24和32 ）影响不大μm） 是的。此外，光斑大小和等离子体条件对精密度、准确度和不确定度的影响很大程度上取决于分析物的灵敏度。

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我们的目的是建立6种成分（5-咖啡酰奎宁酸，3-咖啡酰奎尼酸，4-咖啡酰奎宁酸，1,3-二咖啡酰奎尼酸，3,4-二咖啡酰奎尼酸，4,5-二咖啡酰奎尼酸）的定量分析方法在毛蒿（茵陈），并通过使用HPLC加上DAD其汤。此外，六个组分的转化路径在汤准备处理进行了分析。分析方法中的线性度，灵敏度，精度，可重复性和恢复方面得到充分验证，并用于评估在茵陈汤六种组分的转化趋势和定量分析。6种组分的含量茵陈药草和茵陈汤加减变化很大，并有他们之间有着不可分割的内在联系。据推测3-咖啡酰奎宁酸异构化生成5-咖啡酰奎宁酸和4-咖啡酰奎宁酸。类似地，1,3-二咖啡酰奎尼酸和3,4-二咖啡酰奎尼酸是由4,5-二咖啡酰奎尼酸的异构化制备。总之，这项研究提供了茵陈汤的质量控制的化学基础，并在煎煮选择标记的变化可以给我们一些新的视角来研究物质和药物疗效之间的关系。

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多毛素和多毛素是从中药中提取的两种生物碱单体钩藤，其中有药理作用，如抗高血压，抗感染，保护心脏。一个超高性能液相色谱法 - 质谱法，建立了hirsutine的确定和hirsuteine在组织（肝，肾，心脏，脾脏，脑，和肺），以及它们的吸收，分布和代谢研究关于提供信息的药理作用机制。UPLC BEH C18 column (2.1  mm × 100  mm, 1.7 μm） 用于色谱分离。流动相为乙腈-0.1%甲酸，梯度洗脱，总运行时间为4 min，正离子模式为电喷雾，多反应监测（MRM）模式为定量。采用乙腈沉淀法去除蛋白处理的小鼠血浆和组织匀浆样品。在2-5000 ng/g浓度范围内，组织中的多毛素和多毛素呈良好的线性关系([R > 0.995), and the lower limit of quantification was 2 ng/g. In the plasma and liver tissues, the interday and intraday precision of hirsutine and hirsuteine was less than 15%, the accuracy was between 90.9% and 110.1%, and the average recovery was better than 73.0%. The matrix effect was between 86.2% and 104.7%. The results showed that the precision, accuracy, recovery, and matrix effects meet the requirements for the study on the distribution of hirsutine and hirsuteine. After intraperitoneal administration of 10 mg/kg hirsutine and hirsuteine in mice, the distribution levels were highest in liver and kidney tissues, followed by the spleen and lung. Hirsutine and hirsuteine were low in brain tissue, but had obvious distribution, suggesting that they may pass through the blood-brain barrier.

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