Ty - Jour A2 - Comi，Giuseppe Au - Mir，Raies A. Au - Brunelle，Brian W. Au - Alt，David P. Au - Arthur，Terrance M. Au - Kudva，Indira T PY - 2020DA - 2020/04/ 14 TI - 超级大肠杆菌O157：H7有可能增加持续持续鳞状上皮细胞和抗生素抗性SP - 2368154 VL - 2020 AB - 超级碎纸 大肠杆菌O157：H7（O157）≥10^4.形成菌落的单位/ g粪便。我们最近证明，超型O157（SS-O157）菌株，SS-17，异常结垢（RAJ）鳞状上皮（RSE）细胞的鳞状上下鳞（RSE）细胞，其在该网站上以更大的数量有助于SS-O157持久性，从而增加粪便O157负载表征超空白现象。为了验证这是否是任何SS-O157的签名依从性，我们测试了其他SS-O157隔离（ N = 101; each from a different animal) in the RSE cell adherence assay. Similar to SS-17, all 101 SS-O157 exhibited aggregative adherence on RSE cells, with 56% attaching strongly (>10 bacteria/cell; hyperadherent) and 44% attaching moderately (1–10 bacteria/cells). Strain typing using Polymorphic Amplified Typing Sequences (PATS) analysis assigned the 101 SS-O157 into 5 major clades but not to any predominant genotype. Interestingly, 69% of SS-O157 isolates were identical to human O157 outbreak strains based on pulsed field gel electrophoresis profiles (CDC PulseNet Database), grouped into two clades by PATS distinguishing them from remaining SS-O157, and were hyperadherent on RSE cells. A subset of SS-O157 isolates ( N = 53) representing different PATS and RSE cell adherence profiles were analyzed for antibiotic resistance (AR). Several SS-O157 (30/53) showed resistance to sulfisoxazole, and one isolate was resistant to both sulfisoxazole and tetracycline. Minimum inhibitory concentration (MIC) tests confirmed some of the resistance observed using the Kirby–Bauer disk diffusion test. Each SS-O157 isolate carried at least 10 genes associated with AR. However, genes directly associated with AR were rarely amplified: AAC（3）-IV在2个分离物中， 苏2/3分离物，和 TETB.在一个孤立中。整容酶基因， ㈡与基于整合子的AR采集/传输相关联的，在92％的SS-O157分离液中识别出来。我们的结果表明，SS-O157分离物可能在牛RAJ处潜在持续更长时间，但对临床抗生素具有有限的抗性。SN - 1687-918X UR - HTTPS://Doi.org/10.1155/2020/2368154 Do - 10.1155 / 2020/21368154 JF - 国际微生物学杂志PB - Hindawi Kw - ER -