TY - JOUR A2 - Ierapetritou, M. G. AU - Sakharov, Vladimir AU - Baykov, Sergey AU - Konstantinova, Irina AU - Esipov, Roman AU - Dorogov, Mikhail PY - 2015 DA - 2015/11/16 TI - An Efficient Chemoenzymatic Process for Preparation of Ribavirin SP - 734851 VL - 2015 AB - Ribavirin is an important antiviral drug, which is used for treatment of many diseases. The pilot-scale chemoenzymatic process for synthesis of the active pharmaceutical ingredient Ribavirin was developed with 32% overall yield and more than 99.5% purity. The described method includes the chemical synthesis of 1,2,4-triazole-3-carboxamide, which is a key intermediate and enzyme-catalyzed transglycosylation reaction for preparation of the desired product. 1,2,4-Triazole-3-carboxamide was synthesized from 5-amino-1,2,4-triazole-3-carboxylic acid by classical Chipen-Grinshtein method. Isolated from E. сoliBL21(DE3)/pERPUPHHO1 strain the purine nucleoside phosphorylase was used as a biocatalytical system. All steps of this process were optimized and scaled. SN - 1687-806X UR - https://doi.org/10.1155/2015/734851 DO - 10.1155/2015/734851 JF - International Journal of Chemical Engineering PB - Hindawi Publishing Corporation KW - ER -