TY - Jour A2 - Lubman，David M. Au - Li，双龙Au - 朱，永良奥义 - 张，易奥·刘，舒汉奥王，翔泥 - 邱，湘军PY - 2020 DA - 2020 / 03/31 - 大鼠等离子体中enaSidenib的UPLC-MS / MS方法的开发和验证SP - 5084127 VL - 2020 AB - 我们的研究，是一款简单的UPLC-MS/ MS方法，提出并确认用Diazepam作为内标（是），以确定大鼠等离子体中Enasidenib的浓度。当准备样品时，我们使用乙腈进行蛋白质沉淀。使用梯度洗脱方法，流动相是乙腈和0.1％甲酸。用态度使用了Diazepam。我们使用Acquity Uplc Beh C18列来分隔Enasidenib。在正离子电喷雾电离（ESI）源条件下，通过多次反应监测（MRM）监测enaSidenib的传质对 m / z.474.2÷456.1和 m / z.474.2÷267.0，并且是大规模转移对 m / z.285.0÷154.0。enasidenib有良好的线性度（ R. ² = 0.9985) in the concentration range of 1.0–1000 ng/mL. Besides, the values of intraday and interday precision were 2.25–8.40% and 3.94–5.46%, respectively, and the range of the accuracy values varied from −1.44 to 2.34%. Matrix effect, extraction recovery, and stability were compliant with FDA approval guidelines in terms of bioanalytical method validation. We had established a new method that had been applied to the pharmacokinetic study of enasidenib in rats. SN - 1687-8760 UR - https://doi.org/10.1155/2020/5084127 DO - 10.1155/2020/5084127 JF - International Journal of Analytical Chemistry PB - Hindawi KW - ER -