TY - JUR A2 - Cheng，Cheng-i Au - Liang，Jun-Xian Au - Liao，Xuan Au - Li，Sheng-Hong Au - Jiang，肖奥 - 李，Ze-Hua Au - Wu，Yin-Di Au -肖，李玲奥谢，广辉·奥 - 宋，建兴AU - 刘，洪伟PY - 2020DA - 2020 / 12/21 - 2020/12/21 - 2020/12/21 - 来自脂肪衍生的间充质干细胞的外泌体Photogged Rat Skin SP-6406395 VL - 2020 AB - 脂肪衍生的干细胞（ADSCs）被记录为可能的皮肤复兴的候选者。然而，adsc衍生的外来体对像素皮肤的影响仍然被完全阐明。本研究旨在确定Adsc衍生的外来体对肌肤皮肤的抗衰率效应。人体ADSC与健康女性的脂肪组织分离，体外培养。然后，从培养的ADSC中提取外来，通过超速离心纯化，并通过使用透射电子显微镜检查细胞形态来验证和鉴定特定生物标志物。同时，选择最佳外出浓度和治疗时间。通过对Sprague-Dawley大鼠进行紫外线B辐射来创建的Photoged皮肤模型。外泌体在单一治疗剂量中注射到拍摄皮肤中。 The thickness of the epidermis and dermis was observed by HE staining. The relative mRNA expression of type I collagen, type III collagen, and matrix metalloproteinases (MMP-1 and MMP-3) was determined by real-time PCR. In the rat model of photoaged skin, the injected exosomes markedly decreased the epidermal thickness and increased the dermal thickness of the photoaged skin 7 days after treatment. Moreover, the proportion of the stratum corneum of the epidermis was decreased. Furthermore, real-time RT-PCR showed that the mRNA expression of type I collagen was increased and that of type III collagen, MMP-1, and MMP-3 was decreased. Our results demonstrate that ADSC-derived exosome treatment could significantly improve skin photodamage and that ADSC-derived exosomes may be a potential agent for photoaged skin treatment. SN - 2314-6133 UR - https://doi.org/10.1155/2020/6406395 DO - 10.1155/2020/6406395 JF - BioMed Research International PB - Hindawi KW - ER -